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. 2014 Jun 24;65(17):4849–4861. doi: 10.1093/jxb/eru259

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — The transcript levels of OsNRAMP5 in different tissues of rice plants. (A) The transcript levels of OsNRAMP5 in 27 samples representing different organs or tissues at different developmental stages of the Minghui 63 (MH63) and Zhenshan 97 (ZS97) based on signal values from Wang et al. (2010). Numbers from 1 to 27 represent the following 27 samples: (1) calli, 15 days after subculture; (2) calli, screening stage; (3) calli, 5 days after regeneration; (4) seed, 72h after imbibition; (5) embryo and radicle after germination; (6) leaf and root at three-leaf stage; (7) root, seedling with 2 tillers; (8) shoot, seedling with 2 tillers; (9) sheath, young panicle at stage 3; (10) leaf, young panicle at stage 3; (11) leaf, 4–5cm young panicle; (12) flag leaf, 5 days before heading; (13) flag leaf, 14 days after heading; (14) sheath, 4–5cm young panicle; (15) stem, 5 days before heading; (16) stem, heading stage; (17) young panicle at stage 3; (18) young panicle at stage 4; (19) young panicle at stage 5; (20) panicle, 4–5cm young panicle; (21) stamen, one day before flowering; (22) panicle, heading stage; (23) spikelet, 3 days after pollination; (24) hull, one day before flowering; (25) endosperm, 7 days after pollination; (26) endosperm, 14 days after pollination; (27) endosperm, 21 days after pollination. (B) Quantitative RT-PCR analysis of the expression pattern of OsNRAMP5 in various tissues from Zhonghua 11 (ZH11). B, leaf blade; C1, culm I; C2, culm II; C3, culm III; P, panicle; Sh, sheath; R, root. (C) Quantitative RT-PCR analysis of the OsNRAMP5 transcriptional level in the third leaf of Zhonghua 11 seedling at the two-to-six-leaf stages. (D) The expression level of OsNRAMP5 in different leaves of rice plants at different Mn supplies; the 1st leaf is the oldest of the four leaves from the tiller and the 4th leaf is not fully expanded. (E) The expression pattern of OsNRAMP5 in different regions of the 4th leaf. Data are means ± SD of three biological replicates in real-time PCR.