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. 2014 Jun 24;65(17):4849–4861. doi: 10.1093/jxb/eru259

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — The expression pattern of OsNRAMP5. (A–L) In situ hybridization of OsNRAMP5. (A–C) Transverse section of roots. (A) The elongation zone of root. (B) The mature zone of root. (C) Magnified image of part of B. (D) Transverse section of unexpanded leaf. (E) Magnified image of part of D. (F) Transverse section of a young tiller. (G) Magnified image of part of F. (H) Transverse section of culm. (I) Transverse section of expanded leaf. (J) Transverse section of the border region of the node. (K) Magnified image of part of J. (L) A negative control detected by the sense probe. (M–Z7) Expression pattern of OsNRAMP5 detected in plants transformed with the construct OsNRAMP5-promoter:GUS by histochemical straining of GUS activity. (M–Z1) The localization of OsNRAMP5 in leaves and roots of a plant cultivated hydroponically for two weeks. (M) The expanded young leaf. (N) Magnified image of part of M. (O) Transverse section of leaf shown in M. (P) Enlarged view of a vascular bundle from O. (Q) The tip of root. (R) The elongation zone of root. (S, T) The basal (S) and the mid (T) mature zone of root. (U) The lateral roots. (V) The transverse section of R. (W) The transverse section of T. (X) The transverse section of a small lateral root described in U. (Y) Magnified image of part of V. (Z1) Magnified image of part of W. (Z2–Z7) The localization of OsNRAMP5 in hulls. (Z2) The hull, 5 days before heading. (Z3) The hull, 2 days before heading. (Z4, Z5) The hull at heading stage. (Z6, Z7) The hull and endosperm, 21 days after pollination. X, xylem region of regular vascular bundles; XD, xylem region of diffuse vascular bundles; P, phloem region of regular vascular bundles. Scale bars (A–L) =100 µm.