Fig. 3.

RBOHDs form clusters in the PM. (A) Representative transmission electron micrographs of BY-2 cell PM vesicle labelled with anti-RBOHD antibody and secondary IgG coupled to GPs of 5nm (GAR). Scale bar = 100nm. (B) Labelling characteristics of PM vesicles. Vesicles were treated or not with MβCD, then labelled with anti-RBOHD and either GAR or PAGs of 5nm (n, number of experiments). (C) Ripley’s K-function analysis of RBOHD distribution on PM vesicles. K(r) (y axis) is the average number of particles lying at a distance less than r (x axis) from a typical particle, normalized by the mean particle density. K(r) values displayed above simulation intervals for a completely random (Poisson) point pattern indicate an aggregated pattern. Black line, sample K(r); dotted line, theoretical Poisson K(r); grey area, 99% Poisson simulation interval.