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. 2014 Jun 24;65(17):5049–5062. doi: 10.1093/jxb/eru270

Fig. 6.

Fig. 6.

Maintenance of GST stability by MSRB7 in vivo. (A, B) Expression patterns of GSTF2 and GSTF3. Real-time PCR analysis of GSTF2 and GSTF3 transcripts in 10-d-old B7Ox, B7i, and 1301 plants treated with 10 μM MV for 8h. (C) Immunoblotting of GSTF2/3 in aerial parts and roots. Ten-day-old 1301, B7Ox, and B7i seedlings were pre-treated with 10 μM MV for 8h, followed by treatment with 0.5mM CHX for 0–36h. GSTF2/3 was detected using an anti-GSTF2/3 antibody. Protein stained with Coomassie Brilliant Blue (CBB) was used as a protein loading control. (D, E) Relative expression of GSTF2/3. The relative amounts of GSTF2/3 in the aerial parts and roots were determined using immunoblot analysis, and quantified using G:Box iChemi XL (Syngene). Data were analysed statistically using Duncan’s test and different letters indicate significant differences at P<0.05. (F) Total GST activity of MSRB7 transgenic plants. Ten-day-old 1301, B7Ox, and B7i seedlings were pre-treated with 10 μM MV for 8h followed by treatment with 0.5mM CHX for 0–48h. GST activity was measured. Data represent the means±SD (n=10) of three independent experiments.