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. 2014 Jul 4;65(17):5093–5107. doi: 10.1093/jxb/eru274

Fig. 1.

Fig. 1.

RT–PCR examination of the transcripts of novel cystatin family genes in tobacco. cDNA prepared from root, stem, leaf, pollen, anther, and seeds at stage 1, 5, and 9 were selected as templates for PCR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the control. The nine stages of seed development were divided according to the corresponding stages of embryogenesis in tobacco (Zhao et al., 2013).