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. 2014 Jul 4;65(17):5093–5107. doi: 10.1093/jxb/eru274

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Phylogenetic relationships of the cystatins from Nicotians tabacum, Arabidopsis thaliana, Glycine max, Oryza sativa, Hordeum vulgare, and Solanum lycopersicum. The tree was calculated with Phylip Ver. 3.68 software using the Protpars method. The numbers at the nodes indicate the bootstrap values. Accession numbers of the protein sequences used in this analysis were as follows: A. thaliana AtCYS1 (AT5G12140), AtCYS2 (AT2G31980), AtCYS3 (AT3G12490), AtCYS4 (AT4G16500), AtCYS5 (AT5G47550), AtCYS6 (At3g12490), AtCYS7 (At5g05110); G. max GmCYS1 (ACU14306), GmCYS2 (CAI84599), GmCYS3 (CAI84598), GmCYS4 (BAA19610), GmCYS5 (ACU19522), GmCYS6 (ACU14962), GmCYS7 (CAI84604), GmCYS8 (CAI84601); O. sativa OsCYS1 (Os01g58890), OsCYS2 (Os05g41460),OsCYS3 (Os05g33880), OsCYS4 (Os01g68660), OsCYS5 (Os01g68670), OsCYS6 (Os03g11180), OsCYS7 (Os03g11170), OsCYS8 (Os03g31510), OsCYS9 (Os03g11160), OsCYS10 (Os04g28250), OsCYS11 (Os09g08100), OsCYS12 (Os01g16430); H. vulgare HvCYS1 (Y12068), HvCYS2 (AJ748337), HvCYS3 (AJ748338), HvCYS4 (AJ748344), HvCYS5 (AJ748340), HvCYS6 (AJ748341), HvCYS7 (AJ748345), HvCYS8 (AJ748343), HvCYS9 (AJ748339), HvCYS10 (AJ748342), HvCYS11 (AJ748346), HvCYS12 (AJ748347), HvCYS13 (AJ748348); and S. lycopersicum SlCYS1 (AAF23126), SlCYS2 (ABG23376).