Fig. 4.

Relative transcript abundance of DXS and HDR isoforms in organs of adult PS (top) or Dul (bottom) plants. cDNA loading was normalized using three distinct reference genes (APT1, ACT2/8, and CYC). RNA was extracted from three individual plants for each organ (n = 3) and assayed by quantitative RT-PCR using SYBR Green as three technical replicates each. Relative fold-change calculations were performed using the ΔΔCt model (Livak and Schmittgen, 2001) by normalizing all measurements to the median gene expression in the leaf for all DXS and HDR genes. The dashed line signifies a discontinuity in the vertical axis. Error bars indicate the standard error.