Fig. 8.

Alignment of selected type I and II DXS sequences. The alignment was created using BLOSSUM62 with a gap-opening penalty of 10 and a gap-extension penalty of 0.05. It includes the presumed mature peptide only (transit peptides were manually curated based on an alignment of the full-length peptide). Residues shaded in black are absolutely conserved while those in grey are conserved among 70% of sequences analysed. The solid bar indicates a deletion in type III DXS sequences. Asterisks indicate residues identified as critical for catalysis in the E. coli protein (Xiang, et al. 2007). The first two letters of each sequence indicates the corresponding species: Aa, Artemisia annua; Mt, Medicago truncatula; Cm, Cucumis melo; Zm, Zea mays; At, Arabidopsis thaliana; Pa, Picea abies; Ec, Escherichia coli; Cr, Catharanthus roseus; CmDXS3, melon DXL.