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Immunoblot detection of WWOX protein in human tissues. Top panel: 20 μg of total protein lysates from each tissue were resolved onto an Immobilon membrane analyzed with anti-WWOX antibody. Full length WWOX protein is ~46 kDa. Bottom panel: Detection of β-actin was performed to determine equal loading of protein from each tissue. The membrane was stripped after WWOX detection and washed extensively before immunodetection with anti-actin antibody
