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. 2014 Aug 26;9(8):e103743. doi: 10.1371/journal.pone.0103743

Figure 5. Coase treatment or cholestenone impairs fibroblast migration in a wound-healing assay.

Figure 5

A) Confluent HDFs were treated with coase (10 U/ml; 1 h), MBCD (0.5 mM; 5 min), or mock treated (ctrl) and collected for lipid extraction at the indicated time points. Cholesterol and cholestenone amounts were determined; n = 8–16. B) HDFs were treated as in A, and wounded with a pipette tip. Cells were fixed at the indicated time points, stained with Alexa568-phalloidin and imaged. Scale bars 20 µm. C) Cell migration area was quantified at 22 h post treatments; n = 11–15 fields. D) HDFs were incubated with MBCD-cholestenone (MB-CN) complex, collected for lipid analysis and cholesterol and cholestenone amounts were determined; n = 6–12. E) HDFs were treated with MB-CN complex as in D, wounded with a pipette tip and incubated in serum-free buffer for 22 h. Cells were stained with Alexa568-phalloidin and the wound area was imaged.