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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Nat Chem Biol. 2014 Feb 16;10(4):298–304. doi: 10.1038/nchembio.1455

Figure 2. Selective inhibition of USP1 by ML323 revealed by DUB profiling.

Figure 2

(a,b) Immunoblot analysis using HA-specific antibody (a) or antibodies to USP1, USP8, USP7, CYLD, USP5, USP14, UCH-L3 and UCH-L1 (b) of HEK293T cells that were treated with ML323 (0.5, 5 or 50 μM) or DMSO in the presence of 100 μM cisplatin, lysed, labeled with HA-Ub-VME and separated by SDS-PAGE. α-tubulin served as a loading control. The full immunoblot gels for this figure are shown in Supplementary Figure 13.