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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Nat Chem Biol. 2014 Feb 16;10(4):298–304. doi: 10.1038/nchembio.1455

Figure 4. The effect of inhibiting USP1-UAF1 by ML323 in the TLS and FA pathways.

Figure 4

(a,b) Colony-forming assay of XPV cells (a) and XPV + Polη cells (b) irradiated with UV alone (black) or a combination of UV and 20 μM ML323 (red). The data represent the mean ± s.d. of three independent experiments. (c,d) Cytotoxicity assay of PD20 cells (c) and PD20 + D2 cells (d) treated with cisplatin alone (black), ML323 (blue) or a combination of cisplatin with ML323 at a ratio of 1:4 (red). The data represent the mean ± s.d. of three independent experiments. (e) Colony-forming assay of PD20 cells irradiated with UV alone (black) or a combination of UV and 20 μM ML323 (red). The data represent the mean ± s.d. of three independent experiments. (f) Comparison of FANCD2 foci formation in U2OS cells treated with 30 μM ML323, 5 μM cisplatin or a combination of 30 μM ML323 and 5 μM cisplatin. Scale bars, 10 μm. DAPI, 4′,6-diamidino-2-phenylindole.