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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biomaterials. 2014 Jun 24;35(29):8348–8356. doi: 10.1016/j.biomaterials.2014.06.018

Figure 1. Distinct responses of PKA activation upon ISO stimulation in HMSCs cultured on substrates with different stiffness.

Figure 1

(A) The cells expressing ECFP-AKAR2-YPet reporter show the FRET ratio changes before and after treatment with a β-AR agonist, ISO, on substrates with different stiffness. Blue and red colors represent low and high FRET ratios, respectively. (B, C) Time courses and bar graphs indicate the average of normalized FRET/ECFP emission ratio changes of the reporter from multiple cells in response to ISO (n=6-10, **P<0.01 and ***P<0.001).