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. 2014 Aug 26;9(8):e105929. doi: 10.1371/journal.pone.0105929

Figure 3. Knock-down of uPAR expression in AT84 cells.

Figure 3

shRNA knock-down of Plaur in AT84 cells. A: Flow chart showing the generation of the single cell clones. B: Western blot analysis of whole cell lysates from the single cell clones stably transfected with either shRNA-constructs (shRNA 3 = sh3, shRNA 4 = sh4 or shRNA 5 = sh5) targeting Plaur or constructs containing non-target shRNA (NT) or the empty vector (EV). uPAR was detected using a polyclonal anti-murine uPAR antibody (AF534). Re-probing for β-actin was used as a loading control. Images were cropped, as no additional bands were detected in the blot.