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. 2014 Aug 26;9(8):e105929. doi: 10.1371/journal.pone.0105929

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© 2014 Magnussen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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ZBF-fixed leiomyoma tissue was sectioned and analysed for the presence of gelatinolytic activity using DQ-gelatin in situ zymography. Gelatinolytic activity is seen as green fluorescence, nuclei are stained blue with DAPI. Representative confocal images of cells expressing either low- (EV1-sh3, left panels) or high (uPAR1-NT, right panels) levels of uPAR invading the ex vivo leiomyoma tissue. The upper panels show gelatinolytic activity in the tissue, while the lower panels show tissue sections treated with the metalloproteinase inhibitor EDTA.