Figure 5. Validation of the Microarray data by RT-PCR and ChIP.

(A) Chromatin Immunoprecipitation studies show that BRG1 binds to the promoter of these genes, and regulates their expression. (B) RT-PCR showing that treatments with 5-aza-2′-deoxycytidine (5-Aza) induces expression of some of the identified BRG1 target genes in SW13 cells, which lack endogenous BRG1 expression.