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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mech Dev. 2013 Oct 23;131:86–110. doi: 10.1016/j.mod.2013.09.005

Table 1.

RNA-Seq analysis identified 190 differentially expressed genes in the Sip1 cKO at E15.5. A value greater than two was used as a cutoff for the un-normalized wild type reads per kilobase per million (RPKM) and Sip1 cKO RPKM means as well as the difference between these means. After normalization, the fold change was calculated and reported here in addition to the p-value (cutoff p < 0.05) and false discovery rate (FDR) p-value correction. The final column (column 8) identifies the expression pattern of each gene during normal development from E10.5 to E12.5 as one of the following groups: significantly downregulated (p < 0.05) in the E10.5 lens compared to the E12.5 lens; significantly upregulated (p < 0.05) in the E10.5 lens compared to the E12.5 lens; not significantly expressed (p > 0.05, detection p-value) in the E10.5 and E12.5 lens as determined by Affymetrix microarrays; not found in the Affymetrix dataset; and expressed but levels are not significantly different between E10.5 and the E12.5 lens.

Gene ID Gene Name Fold Change p-value FDR p-value correction WT RPKM means Sip1cKO RPKM means Normal expression change E10.5 to E12.5
Prr15 proline rich 15 ? 1.75E-05 1.56E-04 0.02 2.22 not significantly expressed
Ucma upper zone of growth plate and cartilage matrix associated 182.3 0 0 0.06 14.93 not significantly expressed
Adamts15 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15 125.3 0 0 0.02 2.24 downregulated
Mt1 metallothionein 1 81.6 0 0 0.92 87.15 downregulated
Aass aminoadipate-semialdehyde synthase 76.6 0 0 0.14 11.85 downregulated
Cxcl14 chemokine (C-X-C motif) ligand 14 58.1 0 0 0.08 5.08 downregulated
Sstr1 somatostatin receptor 1 50.0 0 0 0.08 4.67 not significantly expressed
Krt8 keratin 8 48.1 0 0 0.07 5. 3 3 downregulated
Pde6b phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide 42.5 0 0 0.28 13.90 not significantly expressed
Sntg2 syntrophin, gamma 2 40.5 0 0 0.08 3.64 not significantly expressed
Igfbp2 insulin-like growth factor binding protein 2 37.8 1.58E-09 2.40E-08 0.70 30.86 downregulated
Tnnt1 troponin T1, skeletal, slow 37.3 0 0 0.23 10.12 downregulated
Aldh1a3 aldehyde dehydrogenase family 1, subfamily A3 34.7 0 0 0.16 6.38 downregulated
Dlk1 delta-like 1 homolog (Drosophila) 33.0 8.06E-11 1.41E-09 0.27 10.67 downregulated
Atp7b ATPase, Cu++ transporting, beta polypeptide 28.5 0 0 0.10 3.18 upregulated
Igfbpl1 insulin-like growth factor binding protein-like 1 25.3 0 0 0.97 28.98 not significantly expressed
Plxna2 plexin A2 23.2 0 0 0.38 10.13 downregulated
Best3 bestrophin 3 20.9 0 0 0.10 2.27 not found
Nlgn1 neuroligin 1 16.2 0 0 0.11 2.14 unchanged
Lgsn lengsin, lens protein with glutamine synthetase domain 14.4 0 0 0.06 3.63 not found
Enc1 ectodermal-neural cortex 1 14.3 0 0 0.81 13.73 downregulated
Ces1e carboxylesterase 1E 14.1 0 0 0.65 10.98 not significantly expressed
Uchl1 ubiquitin carboxy-terminal hydrolase L1 14.0 0 0 147.49 2086.29 upregulated
Rgs4 regulator of G-protein signaling 4 11.5 0 0 0.62 8.67 not significantly expressed
Entpd3 ectonucleoside triphosphate diphosphohydrolase 3 11.2 0 0 0.40 5.47 unchanged
Dkk1 dickkopf homolog 1 (Xenopus laevis) 10.3 0 0 0.25 2.95 downregulated
Myh7b myosin, heavy chain 7B, cardiac muscle, beta 10.0 0 0 5.11 58.64 not significantly expressed
Crygs crystallin, gamma S 9.1 0 0 416.21 3724.74 upregulated
Acss2 acyl-CoA synthetase short-chain family member 2 9.0 0 0 0.55 6.73 downregulated
Chrna4 cholinergic receptor, nicotinic, alpha polypeptide 4 8.8 0 0 1.84 19.15 unchanged
Ptprcap protein tyrosine phosphatase, receptor type, C polypeptide-associated protein 8.6 0 0 0.24 2.46 not significantly expressed
Ptprt protein tyrosine phosphatase, receptor type, T 7.6 0 0 0.41 3.80 downregulated
Krt18 keratin 18 7.5 0 0 0.58 5.27 downregulated
Trim55 tripartite motif-containing 55 7.5 0 0 0.55 4.92 not found
Epn3 epsin 3 7.2 0 0 1.98 17.30 not significantly expressed
Rbm20 RNA binding motif protein 20 7.2 0 0 0.28 2.45 downregulated
Fam46b family with sequence similarity 46, member B 7.0 0 0 1.55 13.59 not significantly expressed
Podn podocan 6.6 0 0 1.55 12.35 upregulated
Slc38a5 solute carrier family 38, member 5 6.0 0 0 1.36 9.91 upregulated
Rgmb RGM domain family, member B 5.8 0 0 1.37 9.69 downregulated
Dsp desmoplakin 5.6 0 0 0.38 2.60 downregulated
Grtp1 GH regulated TBC protein 1 5.4 0 0 2.07 13.07 unchanged
Wnk2 WNK lysine deficient protein kinase 2 5.2 0 0 2.41 14.49 upregulated
Gcg glucagon 4.9 0 0 26.45 152.93 upregulated
Rnase4 ribonuclease, RNase A family 4 4.8 0 0 0.47 2.57 downregulated
Fras1 Fraser syndrome 1 homolog (human) 4.7 0 0 0.52 2.93 downregulated
Hddc3 HD domain containing 3 4.7 0 0 2.45 13.90 downregulated
Lbh limb-bud and heart 4.6 0 0 3.78 20.85 downregulated
Lrrc16a leucine rich repeat containing 16A 4.5 0 0 1.16 6.34 downregulated
Gsta4 glutathione S-transferase, alpha 4 4.5 0 0 3.39 18.47 downregulated
Myb myeloblastosis oncogene 4.4 0 0 2.73 15.30 downregulated
Tdrd9 tudor domain containing 9 4.2 0 0 1.31 6.82 not significantly expressed
Ppp2r2b protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), beta isoform 4.1 0 0 1.20 6.02 unchanged
Plekha7 pleckstrin homology domain containing, family A member 7 3.8 0 0 0.67 3.09 downregulated
Cyr61 cysteine rich protein 61 3.8 2.50E-10 4.14E-09 0.58 2.61 downregulated
Edaradd EDAR (ectodysplasin-A receptor)-associated death domain 3.8 0 0 0.93 4.28 downregulated
Rapgef5 Rap guanine nucleotide exchange factor (GEF) 5 3.8 0 0 1.99 9.12 upregulated
Ckb creatine kinase, brain 3.8 0 0 85.35 357.31 upregulated
Fbxo10 F-box protein 10 3.7 0 0 2.57 11.84 unchanged
Gmpr guanosine monophosphate reductase 3.7 0 0 4.30 19.56 unchanged
Slc8a1 solute carrier family 8 (sodium/calcium exchanger), member 1 3.7 0 0 1.44 6.45 not significantly expressed
Asap2 ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 3.6 0 0 2.42 11.50 unchanged
Cited4 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4 3.6 0 0 3.84 16.94 unchanged
Sh3bp4 SH3-domain binding protein 4 3.5 0 0 0.97 4.15 downregulated
R3hdml R3H domain containing-like 3.5 0 0 52.37 185.59 not found
Whrn whirlin 3.4 0 0 2.00 8.32 unchanged
Adcy9 adenylate cyclase 9 3.4 0 0 1.39 5.76 not significantly expressed
Sox3 SRY-box containing gene 3 3.3 0 0 0.67 2.72 unchanged
Lingo3 leucine rich repeat and Ig domain containing 3 3.3 0 0 3.21 12.69 upregulated
Fam132a family with sequence similarity 132, member A 3.2 0 0 7.96 31.16 unchanged
Cbl Casitas B-lineage lymphoma 3.2 1.13E-13 2.60E-12 1.12 4.35 unchanged
Mypn myopalladin 3.1 0 0 2.72 10.33 not significantly expressed
Prkg1 protein kinase, cGMP-dependent, type I 3.1 0 0 3.14 11.98 upregulated
Pgpep1 pyroglutamyl-peptidase I 3.1 0 0 11.03 40.67 unchanged
Cbx6 chromobox homolog 6 3.1 0.039 0.176 1.26 3.34 unchanged
Rab6b RAB6B, member RAS oncogene family 3.1 0 0 2.12 7.93 not significantly expressed
Apln apelin 3.1 0 0 0.93 3.52 upregulated
Mpp7 membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) 3.0 0 0 1.40 4.95 downregulated
Gpd2 glycerol phosphate dehydrogenase 2, mitochondrial 3.0 0 0 1.31 4.83 unchanged
Zfp111 zinc finger protein 111 3.0 0 0 0.82 3.04 not significantly expressed
Iqgap1 IQ motif containing GTPase activating protein 1 3.0 0 0 6.14 20.86 downregulated
Zbtb4 zinc finger and BTB domain containing 4 2.9 0 0 1.47 5.15 upregulated
Dapl1 death associated protein-like 1 2.9 1.97E-05 1.74E-04 2.91 10.10 unchanged
Spna1 spectrin alpha 1 2.8 0 0 1.44 4.85 unchanged
Itgb8 integrin beta 8 2.8 0 0 0.87 2.95 upregulated
Rassf4 Ras association (RalGDS/AF-6) domain family member 4 2.8 0 0 1.17 3.97 upregulated
Vill villin-like 2.8 0 0 1.26 4.28 unchanged
Polr2b polymerase (RNA) II (DNA directed) polypeptide B 2.8 1.67E-06 1.74E-05 14.31 27.86 downregulated
Igkc immunoglobulin kappa constant 2.7 2.04E-11 3.83E-10 2.39 7.82 unchanged
Btg2 B-cell translocation gene 2, anti-proliferative 2.7 0 0 6.80 22.34 upregulated
Dsc2 desmocollin 2 2.7 8.85E-13 1.89E-11 1.39 4.61 downregulated
Stac2 SH3 and cysteine rich domain 2 2.7 0 0 6.38 20.70 upregulated
Jhdm1d jumonji C domain-containing histone demethylase 1 homolog D (S. cerevisiae) 2.7 0 0 1.83 4.12 unchanged
Magi2 membrane associated guanylate kinase, WW and PDZ domain containing 2 2.7 0 0 3.65 9.31 upregulated
Cmtm7 CKLF-like MARVEL transmembrane domain containing 7 2.6 0 0 7.06 23.48 unchanged
Neo1 neogenin 2.6 0 0 9.54 28.47 unchanged
Snx29 sorting nexin 29 2.6 0 0 1.14 3.55 not significantly expressed
Hes6 hairy and enhancer of split 6 (Drosophila) 2.6 0 0 7.35 22.93 unchanged
Cyp26a1 cytochrome P450, family 26, subfamily a, polypeptide 1 2.5 0 0 1.70 5.32 downregulated
Dusp6 dual specificity phosphatase 6 2.5 0 0 6.56 20.05 downregulated
Tek endothelial-specific receptor tyrosine kinase 2.5 0 0 2.71 8.31 upregulated
Plxna1 plexin A1 2.5 0 0 7.15 14.15 upregulated
Ehd4 EH-domain containing 4 2.5 0 0 1.17 3.62 upregulated
Cyba cytochrome b-245, alpha polypeptide -2.5 1.30E-06 1.37E-05 11.12 5.28 upregulated
mt-Nd6 mitochondrially encoded NADH dehydrogenase 6 -2.5 3.27E-11 6.00E-10 1029.84 462.16 not significantly expressed
Ndufa1 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1 -2.5 6.77E-09 9.58E-08 1097.02 466.07 upregulated
Adc arginine decarboxylase -2.5 8.40E-82 4.22E-80 11.77 5.69 unchanged
Gstp2 glutathione S-transferase, pi 2 -2.6 2.06E-15 5.42E-14 46.96 21.19 upregulated
Lctl lactase-like -2.6 1.35E-13 3.10E-12 31.91 14.24 not significantly expressed
Cxx1b CAAX box 1 homolog B (human) -2.6 4.94E-26 1.78E-24 36.85 27.53 not significantly expressed
Hnrnpa1 heterogeneous nuclear ribonucleoprotein A1 -2.6 2.92E-06 2.95E-05 268.59 212.23 not significantly expressed
Cnih2 cornichon homolog 2 (Drosophila) -2.6 1.21E-15 3.24E-14 23.26 11.02 upregulated
Pacsin3 protein kinase C and casein kinase substrate in neurons 3 -2.6 8.77E-38 3.65E-36 7.90 3.70 unchanged
Apoo apolipoprotein O -2.6 2.37E-40 1.01E-38 17.41 12.01 unchanged
Tppp3 tubulin polymerization-promoting protein family member 3 -2.6 5.90E-46 2.61E-44 33.60 15.59 upregulated
Hmgn3 high mobility group nucleosomal binding domain 3 -2.7 6.35E-19 1.97E-17 170.95 71.81 upregulated
Malat1 metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA) -2.7 4.80E-16 1.32E-14 766.15 318.63 upregulated
Zbtb7b zinc finger and BTB domain containing 7B -2.7 3.88E-26 1.40E-24 35.70 15.71 not significantly expressed
Tecrl trans-2,3-enoyl-CoA reductase-like -2.7 4.85E-52 2.25E-50 3.97 1.83 upregulated
Fgfbp3 fibroblast growth factor binding protein 3 -2.7 1.10E-56 5.19E-55 9.15 4.24 upregulated
Ggct gamma-glutamyl cyclotransferase -2.7 2.30E-22 7.82E-21 25.12 10.99 upregulated
Rpl36a ribosomal protein L36A -2.7 1.65E-16 4.72E-15 172.70 111.20 unchanged
Ceacam10 carcinoembryonic antigen-related cell adhesion molecule 10 -2.7 2.06E-11 3.87E-10 15.39 7.22 not significantly expressed
Cabp5 calcium binding protein 5 -2.7 1.53E-27 5.66E-26 4.78 2.17 not significantly expressed
Pgap2 post-GPI attachment to proteins 2 -2.7 1.32E-71 6.50E-70 25.79 11.09 not found
Pmp22 peripheral myelin protein 22 -2.7 1.26E-68 6.20E-67 103.46 43.53 upregulated
Prdm16 PR domain containing 16 -2.8 4.67E-21 1.55E-19 8.79 3.75 unchanged
Ceacam2 carcinoembryonic antigen-related cell adhesion molecule 2 -2.8 3.59E-23 1.24E-21 7.90 3.47 not significantly expressed
Rnf113a1 ring finger protein 113A1 -2.8 2.46E-28 9.22E-27 141.42 58.35 upregulated
S100a11 S100 calcium binding protein A11 (calgizzarin) -2.9 8.35E-14 1.95E-12 57.95 22.94 upregulated
Ube2v1 ubiquitin-conjugating enzyme E2 variant 1 -2.9 4.30E-10 6.91E-09 43.07 30.23 upregulated
Rnaset2a ribonuclease T2A -2.9 8.01E-56 3.77E-54 122.90 94.01 upregulated
Ngfr nerve growth factor receptor (TNFR superfamily, member 16) -3.0 1.28E-07 1.55E-06 9.53 3.98 upregulated
Rpl17 ribosomal protein L17 -3.0 7.37E-06 6.97E-05 338.70 174.44 upregulated
Nat8l N-acetyltransferase 8-like -3.0 3.68E-184 1.97E-182 4.21 1.74 not found
Tubb2b tubulin, beta 2B class IIB -3.0 4.25E-82 2.14E-80 48.31 19.53 upregulated
Gstm1 glutathione S-transferase, mu 1 -3.1 5.95E-76 2.96E-74 95.78 39.19 upregulated
Tpm2 tropomyosin 2, beta -3.1 4.98E-24 1.74E-22 20.20 7.88 upregulated
Rpl28 ribosomal protein L28 -3.1 2.96E-40 1.26E-38 445.60 227.01 unchanged
Gpx8 glutathione peroxidase 8 (putative) -3.2 6.70E-09 9.49E-08 20.35 8.93 unchanged
Rpl37a ribosomal protein L37a -3.2 6.43E-20 2.06E-18 472.52 328.77 downregulated
Fxyd1 FXYD domain-containing ion transport regulator 1 -3.2 2.91E-11 5.36E-10 12.09 4.67 upregulated
Cyp7b1 cytochrome P450, family 7, subfamily b, polypeptide 1 -3.3 2.50E-66 1.21E-64 3.96 1.47 upregulated
Tubb4 tubulin, beta 4 -3.4 4.18E-21 1.39E-19 17.32 6. 3 9 upregulated
Ccdc23 coiled-coil domain containing 23 -3.4 1.53E-23 5.32E-22 76.36 27.30 upregulated
Pla2g7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) -3.4 0 0 219.38 73.90 upregulated
Cyb5r2 cytochrome b5 reductase 2 -3.4 5.52E-79 2.76E-77 3.79 1.38 not significantly expressed
Tubb2a tubulin, beta 2A class IIA -3.4 5.69E-46 2.52E-44 95.51 39.86 upregulated
Krt40 keratin 40 -3.4 4.00E-28 1.49E-26 3.37 1.21 not found
Wdr41 WD repeat domain 41 -3.4 1.27E-247 6.85E-246 14.38 5.00 upregulated
Elovl4 elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4 -3.5 2.07E-88 1.06E-86 4.01 1.40 upregulated
Cpt1a carnitine palmitoyltransferase 1a, liver -3.6 6.95E-20 2.22E-18 13.96 4.74 upregulated
Rps29 ribosomal protein S29 -3.6 6.61E-39 2.78E-37 595.98 313.83 unchanged
Slc46a3 solute carrier family 46, member 3 -3.7 3.53E-100 1.83E-98 11.63 3.95 not significantly expressed
Syt5 synaptotagmin V -3.7 4.03E-61 1.93E-59 5.24 1.74 not significantly expressed
Rpl38 ribosomal protein L38 -3.7 3.89E-55 1.82E-53 496.75 278.37 unchanged
Rps28 ribosomal protein S28 -3.9 6.67E-18 2.00E-16 315.53 106.45 unchanged
Trpc6 transient receptor potential cation channel, subfamily C, member 6 -3.9 2.78E-67 1.35E-65 22.39 6.95 upregulated
Dnase2b deoxyribonuclease II beta -3.9 2.98E-59 1.42E-57 28.06 8.67 not significantly expressed
Tmprss11e transmembrane protease, serine 11e -4.1 5.82E-120 3.05E-118 9.73 2.93 not significantly expressed
Rbp3 retinol binding protein 3, interstitial -4.2 1.32E-10 2.25E-09 6.69 1.97 upregulated
Stx11 syntaxin 11 -4.3 2.22E-44 9.72E-43 18.36 5.30 not significantly expressed
Rpl11 ribosomal protein L11 -4.3 4.53E-31 1.76E-29 291.83 155.91 unchanged
Dpysl2 dihydropyrimidinase-like 2 -4.4 1.39E-79 6.95E-78 32.94 8.78 downregulated
Arl5c ADP-ribosylation factor-like 5C -4.4 1.44E-04 1.11E-03 3.07 0.85 not found
Aebp1 AE binding protein 1 -4.5 4.85E-219 2.61E-217 6.79 1.87 upregulated
Rpl19 ribosomal protein L19 -4.5 5.80E-10 9.20E-09 338.37 299.04 unchanged
Pgm5 phosphoglucomutase 5 -4.5 1.65E-283 8.91E-282 32.89 8.64 upregulated
Myo7b myosin VIIB -4.5 2.33E-35 9.46E-34 20.19 5.25 upregulated
Kifc3 kinesin family member C3 -4.7 4.80E-36 1.96E-34 4.16 1.09 upregulated
Faim2 Fas apoptotic inhibitory molecule 2 -4.7 7.62E-85 3.87E-83 4.56 1.19 not significantly expressed
Birc7 baculoviral IAP repeat-containing 7 (livin) -5.0 1.04E-36 4.26E-35 37.34 9.16 not significantly expressed
Hba-x hemoglobin X, alpha-like embryonic chain in Hba complex -5.0 2.44E-04 1.81E-03 6.70 1.64 unchanged
Pappa pregnancy-associated plasma protein A -5.0 2.13E-40 9.09E-39 6.03 1.43 not significantly expressed
Rcan2 regulator of calcineurin 2 -5.3 2.11E-40 9.02E-39 10.15 2.36 upregulated
Ermap erythroblast membrane-associated protein -5.3 3.66E-305 1.99E-303 13.11 2.99 unchanged
Cend1 cell cycle exit and neuronal differentiation 1 -5.8 3.19E-29 1.21E-27 4.76 1.02 upregulated
Scn11a sodium channel, voltage-gated, type XI, alpha -6.8 9.47E-282 5.11E-280 7.22 1.31 upregulated
Rpl39 ribosomal protein L39 -6.9 3.24E-31 1.26E-29 489.80 259.10 upregulated
Ndufb4 NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 -6.9 1.75E-68 8.56E-67 36.87 28.71 upregulated
Hba-a2 hemoglobin alpha, adult chain 2 -7.2 6.67E-13 1.44E-11 475.53 108.94 not found
Rpl35a ribosomal protein L35A -7.3 6.60E-48 2.98E-46 62.48 13.72 unchanged
Elavl4 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) -7.4 2.01E-97 1.04E-95 2.93 0.49 not significantly expressed
Cnrip1 cannabinoid receptor interacting protein 1 -7.4 7.37E-160 3.93E-158 5.05 0.80 upregulated
Ccdc109b coiled-coil domain containing 109B -8.0 7.80E-19 2.41E-17 2.36 0.35 not significantly expressed
Actn2 actinin alpha 2 -9.5 3.84E-12 7.82E-11 8.77 1.14 not significantly expressed
Stmn4 stathmin-like 4 -10.0 2.27E-65 1.09E-63 2.95 0.34 upregulated
mt-Atp6 mitochondrially encoded ATP synthase 6 -10.6 7.68E-48 3.46E-46 877.78 156.86 not found
Rpl34 ribosomal protein L34 -24.5 7.09E-84 3.60E-82 215.28 98.65 upregulated
Hist1h2af histone cluster 1, H2af -? 2.83E-09 4.17E-08 4.52 0.03 not found