Table 2.

qRT-PCR validation of EMT-related genes at E15.5. None of the genes previously linked to Sip1 are significantly altered at the RNA level when Sip1 is lost. This correlates to the RNA-Seq data.

Gene	Fold Change in Sip1 cKO†	+/- S.D.	Wild Type +/- S.D.	p-value‡
E-cadherin	-1.81	0.44/0.24	1/0.5	0.16
Vimentin	1.1	2.52/0.77	3.87/0.79	0.91
N-cadherin	-1.14	0.66/0.38	0.54/0.35	0.67
αSMA	1.17	1.03/0.55	0.51/0.34	0.63
Snail1	-1.57	0.94/0.38	1.72/0.63	0.28
Egfr	2.4	6.08/1.72	1.47/0.6	0.18
Zeb1	1.49	1.92/0.84	0.79/0.44	0.38

^†calculated in Microsoft Excel, wild type set equal to 1

^‡calculated using nested ANOVA

S.D. – standard deviation