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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mech Dev. 2013 Oct 23;131:86–110. doi: 10.1016/j.mod.2013.09.005

Table 2.

qRT-PCR validation of EMT-related genes at E15.5. None of the genes previously linked to Sip1 are significantly altered at the RNA level when Sip1 is lost. This correlates to the RNA-Seq data.

Gene Fold Change in Sip1 cKO +/- S.D. Wild Type +/- S.D. p-value
E-cadherin -1.81 0.44/0.24 1/0.5 0.16
Vimentin 1.1 2.52/0.77 3.87/0.79 0.91
N-cadherin -1.14 0.66/0.38 0.54/0.35 0.67
αSMA 1.17 1.03/0.55 0.51/0.34 0.63
Snail1 -1.57 0.94/0.38 1.72/0.63 0.28
Egfr 2.4 6.08/1.72 1.47/0.6 0.18
Zeb1 1.49 1.92/0.84 0.79/0.44 0.38

calculated in Microsoft Excel, wild type set equal to 1

calculated using nested ANOVA

S.D. – standard deviation