Table 4.
The gene promoters for a subset of differentially expressed genes, seven of which increased and one that decreased, were scanned for ZEB binding sites using TFSearch (http://www.cbrc.jp/research/db/TFSEARCH.html) using a threshold of 85.0 (default). The number of sites, position (relative to the known +1 transcription start site in the gene promoter), and sequence containing the site are reported. ZEB sites are underlined and CACCTG sites are highlighted in red, while CACCT sites are in black.
| Gene | Number of Sites† | Position‡ | Sequence |
|---|---|---|---|
| K8 | 8 | -134 to -139 | CCTCACCTGAGT |
| -682 to -686 | CTCCACCTAAAG | ||
| -1173 to -1178 | GTCCAGGTGGCT | ||
| -1412 to -1417 | TTGCAGGTGGAA | ||
| -2050 to -2055 | TTTCACCTGAAA | ||
| -2067 to -2071 | ACACACCTAAAT | ||
| -2410 to -2414 | CCACACCTTGAG | ||
| -2433 to -2437 | TCTCACCTTTAC | ||
|
| |||
| K18 | 1 | -1215 to -1219 | TTGAGGTGGG G G |
|
| |||
| Tnnt1 | 1 | -1253 to -1258 | GGCCAGGTGTGA |
|
| |||
| Aldh1A3 | 6 | -1258 to -1263 | TCTCACCTGCAT |
| -1415 to -1419 | CCAAGGTGGGAG | ||
| -1872 to -1876 | GGAAGGTGAGCC | ||
| -2090 to -2095 | ACTCACCTGTAA | ||
| -2108 to -2113 | ATCCACCTGCCT | ||
| -2278 to -2282 | TAGCACCTTAAT | ||
|
| |||
| Dlk1 | 4 | -676 to -680 | AAGCACCTTTAC |
| -1657 to -1661 | GTCCACCTAGAG | ||
| -2071 to -2075 | GCTCACCTCACT | ||
| -2333 to -2338 | GGACAGGTGTGT | ||
|
| |||
| PlxnA2 | 1 | -374 to -379 | TCACAGGTGAAG |
|
| |||
| Dkk1 | 3 | -53 to -57 | CTAAGGTGAGCT |
| -350 to -355 | AGCCACCTGGGC | ||
| -2142 to -2147 | CCTCAGGTGTGG | ||
|
| |||
| Dsp | 3 | -194 to -198 | TCTCACCTCATA |
| -634 to -638 | CTGAGGTGTTTA | ||
| -2413 to -2418 | TGACAGGTGGCA | ||
|
| |||
| Trpc6 | 3 | -403 to -407 | TAAAGGTGGGGA |
| -1435 to -1439 | TAGAGGTGAGTG | ||
| -2246 to -2251 | CATCACCTGTTT | ||
†
found using TFSearch (http://www.cbrc.jp/research/db/TFSEARCH.html)
‡
relative to the known +1 transcription start site in the gene promoter