Figure 4. Morpholino-knockdown efficiency of nr2f1a in zebrafish embryos.

(A) Scheme shows nr2f1a morpholino targeting the pre-mRNA structure of nr2f1a and suggested mis-splicing fragment which can be detected by a nr2f1a_MO_f and nr2f1a_MO_r primer set. (B) cDNA from uninjected controls or nr2f1a morphants (injected with 4 ng morpholino) underwent PCR with primers for the housekeeping control gene (GAPDH), or for the nr2f1a gene. Morphants injected with nr2f1a^e2i2, GAPDH levels are unchanged while the amount of wild-type nr2f1a product (683 bp) got diminished and a new lower molecular weight band appeared (120 bp), representing mis-splicing caused by the morpholino.