Figure 3. Analytical SEC of the HSPB6 10-residue deletion mutants and HSPB1.

100 µl of each protein at 10 mg/ml was loaded on a Superdex 200 10/300 column at 4°C equilibrated in 25 mM HEPES, 150 mM NaCl and 2.5 mM DTT at pH 7.5.