Table 1. Biophysical properties of wild-type HSPB6 and its mutants.
| Construct | Concentration dependenceof apparent MWa(kDa/(mg/ml)) | Hydropathy ofthe NTDb |
| WT | 1.51 | 0.167 |
| ΔN11 | N/A | 0.137 |
| Δ11–20 | 0.81 | 0.209 |
| Δ21–30 | 1.29 | 0.172 |
| Δ31–40 | –0.14 | 0.194 |
| Δ41–50 | 1.92 | 0.128 |
| Δ51–60 | 0.31 | 0.171 |
| Δ61–70 | 3.40 | 0.147 |
| Δ31–35 | –0.01 | 0.218 |
| Δ36–40 | 1.80 | 0.142 |
| Q31A | 1.10 | 0.188 |
| Q31L | 1.87 | 0.194 |
| F33A | –0.21 | 0.159 |
| F33Y | 0.42 | 0.154 |
a
Slope of a linear fit of the calculated MW (determined from calibration with the Molecular Weight kit from GE Healthcare) at three different concentrations.
b
Calculated as the average over the NTD using the hydrophobicity scale according to Eisenberg et al. [53].