Table 2. SAXS data analysis.

Construct	Estimated MW (kDa)		Calculated numberof subunits	Rg (Å)	Concentration(mg/ml)a
	based on I0 of water	based on Porod volume
WT	31.4	38.1	2.2	27.5±1.8	0.61
ΔN11	31.2	39.9	2.5	31.9±2.8	0.65
Δ11–20	25.7	29.5	1.8	25.2±1.1	0.47
Δ21–30	32.8	35.9	2.2	28.7±0.7	0.89
Δ31–40	28.2	32.9	2.0	26.7±2.2	0.79
Δ41–50	34.8	36.6	2.3	28.2±0.7	0.88
Δ51–60	28.3	30.8	1.9	27.3±1.3	0.87
Δ61–70	51.6	56.0	3.5	33.4±0.5	0.77
Δ31–35	30.9	35.3	2.2	27.4±1.3	0.97
Q31A	67.4	71.2	4.2	37.5±0.8	1.37
Q31L	64.7	72.9	4.3	38.0±1.2	1.31
F33A	29.4	38.4	2.3	30.2±1.0	2.32
F33Y	36.8	42.6	1.9	30.9±1.6	1.77

^aThe concentration for each construct at the peak maximum was calculated using the maximum absorbance at 280 nm as it eluted from the column and their calculated absorbance coefficients.