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. 2014 Aug 26;9(8):e105892. doi: 10.1371/journal.pone.0105892

Table 2. SAXS data analysis.

Construct Estimated MW (kDa) Calculated numberof subunits Rg (Å) Concentration(mg/ml)a
based on I0 of water based on Porod volume
WT 31.4 38.1 2.2 27.5±1.8 0.61
ΔN11 31.2 39.9 2.5 31.9±2.8 0.65
Δ11–20 25.7 29.5 1.8 25.2±1.1 0.47
Δ21–30 32.8 35.9 2.2 28.7±0.7 0.89
Δ31–40 28.2 32.9 2.0 26.7±2.2 0.79
Δ41–50 34.8 36.6 2.3 28.2±0.7 0.88
Δ51–60 28.3 30.8 1.9 27.3±1.3 0.87
Δ61–70 51.6 56.0 3.5 33.4±0.5 0.77
Δ31–35 30.9 35.3 2.2 27.4±1.3 0.97
Q31A 67.4 71.2 4.2 37.5±0.8 1.37
Q31L 64.7 72.9 4.3 38.0±1.2 1.31
F33A 29.4 38.4 2.3 30.2±1.0 2.32
F33Y 36.8 42.6 1.9 30.9±1.6 1.77
a

The concentration for each construct at the peak maximum was calculated using the maximum absorbance at 280 nm as it eluted from the column and their calculated absorbance coefficients.