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. 2014 Aug 26;9(8):e106211. doi: 10.1371/journal.pone.0106211

Figure 5. Vg represses th/DIAP1 expression in the wing pouch.

Figure 5

A–B. Expression of the SWH pathway specific th/DIAP1 reporter IAP2B2C-lacZ (red; A′&B′). While IAP2B2C-lacZ are uniform between the anterior and posterior of the pouch in control discs (A), a slight increase in the posterior compartment could be detected in discs where vg activity is lowered by RNAi only in the posterior compartment (B; marked by GFP; green; yellow arrowhead). C–E. Quantification of the posterior compartment increase of IAP2B2C-lacZ expression after vg RNAi knock-down. C. Higher magnification of the image in B′ showing IAP2B2C-lacZ expression in hh-Gal4 vg-RNAi expressing discs. D. Example of the profile of grey levels (reflecting IAP2B2C-lacZ levels), along the yellow box indicated in C. Levels are higher in the posterior compartment expressing the vg-RNAi than in anterior, except for effects at the boundary that are not understood. E. Quantification of the average IAP2B2C-lacZ intensity ratios between equivalent area picked in the posterior (green box in C) and anterior (white box in C) compartments in several discs. While the ratio is at 0.990 in control discs reflecting similar expression levels between the posterior and anterior compartments, this ratio rises to 1.122 in experimental discs showing that IAP2B2C-lacZ expression is 12% higher in the vg depleted compartment. Standard error to the mean is shown; unpaired two-tailed student t-test was performed showing significance with p value = 0.0006 (***).