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. Author manuscript; available in PMC: 2015 Sep 21.
Published in final edited form as: Lab Chip. 2014 Jul 24;14(18):3582–3588. doi: 10.1039/c4lc00676c

Figure 2. Microchip for protein secretion profiling of glioma and macrophage cells at the single-cell level.

Figure 2

(a) Work flow of high-throughput multiplexed single cell secretomic assay to measure glioma, macrophage and their combinations. (b) Optical micrograph of single macrophage (red), glioma (green) and glioma- macrophage co-culture microwells and their corresponding scanned fluorescence images showing the raw data of single cell secretomic measurements. Blue lines are position marks created with immobilized Fluorescein Isothiocyanate labeled bovine serum albumin (FITC–BSA, 488). Red is APC dye-streptavidin (Cy5, 635) for specific protein detection within each microwell.