(A) The position of the NAc region used for quantification is indicated by grey
square at 1.18 mm from Bregma according to Franklin and Paxinos (2008). (B) c-Fos
immunoreactivity in the NAc core of control (AAV-YFP) or AAV-p11 infected mice 2 hrs after
the last saline or cocaine injection of a 10 day treatment. White arrows indicate infected
cells, note strong c-Fos induction after cocaine treatment in YFP infected cells, and
decreased c-Fos induction by cocaine in p11 infected neurons. Scale bar 50 µm. (C)
quantification of c-Fos positive cells within the infected area. Data (means ±SEM
n=6–10 per group) were analyzed using two way ANOVA. Effect of injected virus
F(1, 28)= 9.23, p=0.0055; effect of drug treatment F(1,
28)= 11.46, p=0.0024; interaction F(1, 28)= 0.16, NS.
Post hoc comparison (Tukey HSD test)
**p<0.01.