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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: Biol Psychiatry. 2014 Feb 26;76(10):794–801. doi: 10.1016/j.biopsych.2014.02.012

Figure 4. D1 receptor expressing MSNs mediate the effect of NAc p11 on cocaine CPP.

Figure 4

(A) Scheme of the Cre inducible shRNA expression system used to selectively silence p11 expression. (B) HEK 293 cells were transfected with the indicated constructs and probed for p11 expression 72hrs later. In the presence of Cre lox.sh.p11 construct silences p11 with a similar efficiency than the constitutively active sh.p11. (C) D1-Cre mice injected in the NAc with Cre inducible AAV2-lox.sh.p11 or control AAV2-lox.sh.luc, viral spread at the site of the injection was determined by RFP staining, an adjacent slice was stained for Cre in brown, and p11 in blue. Right panel is a magnification of the double staining in the infected area (white square), white arrows indicate cells expressing Cre and p11 in control injected mice, yellow arrows indicate cells expressing p11 alone and blue arrows indicate cells expressing Cre alone. Scale bar is 50 µm. (d) Ten week old D1-Cre or D2-Cre mice were injected in the NAc with Cre inducible AAV2-lox.sh.p11 or control AAV2-lox.shluc, wt littermates were injected with AAV2-sh.p11 or AAV2-sh.luc 10 weeks later mice were submitted to cocaine (7.5mg/kg) place conditioning as described in the materials and methods section. Data (means ±SEM n=5–10 per group) were analyzed using two way ANOVA. Effect of injected virus F(1, 45)= 5.06, p=0.0301; effect of genotype F(2, 45)= 1.45, NS; interaction F(2, 45)= 0.42, NS. Post hoc comparison (Tukey HSD test) *p<0.05; **p<0.01.