Quantitative polymerase chain reaction analysis of hMSC gene expression. DBM, RBM, and PLG scaffolds seeded with MSCs and collected after 24 hours, 72 hours, 14 days, or 21 days.
ALP:
ap<0.01 compared to DBM at 24/72 hours; bp<0.001 compared to RBM at 24/72 hours; cp<0.01 compared to PLG at 7 days; dp<0.001 compared to DBM at 24/72 hours; ep<0.001 compared to PLG at 14 days; fp<0.001 compared to RBM at 24/72 hours, 14 days; gp<0.001 compared to DBM/PLG at 14 days
IBSP:
ap<0.001 compared to DBM at 24/72 hours, 7days; bp<0.001 compared to PLG at 14 days; cp<0.05 compared to RBM at 14 days; dp<0.001 compared to PLG at 14 days
Runx2:
ap<0.05 compared to DBM at 24
CyclinD1:
ap<0.05 compared to RBM at 72 hours; bp<0.01 compared to PLG at 72 hours; cp<0.05 compared to PLG at 24 hours; dp<0.05 compared to RBM at 24/72 hours; ep<0.01 compared to PLG at 72 hours