Table 1. Brassicaceae Species Included in This Study and Summary Statistics of Transcriptome Sequencing.
| Crucifer Speciesb,c | Chromosome Number (2n)d | Relative 1C Content (pg)e | Relative Genome Size (Mb)e | Sequencing and de Novo Assemblya |
||
|---|---|---|---|---|---|---|
| Raw Data (Mb) | Clean Data (Mb) | Unigenes | ||||
| Brassicaceae lineage I | ||||||
| Armoracia rusticana | 32 | 0.75 | 738.47 | 104.2 | 99.4 | 32,531 |
| Barbarea verna | 16 | 0.63 | 616.78 | 183.0 | 174.0 | 46,149 |
| Capsella bursa-pastoris | 32 | 0.23 | 198.91 | 220.4 | 196.5 | 29,299 |
| Erysimum cheiri | 14 | 0.24 | 210.22 | 260.0 | 137.5 | 20,776 |
| Lepidium densiflorum | 32 | 0.22 | 192.77 | 284.4 | 162.1 | 24,433 |
| Lepidium meyenii | 64 | 0.85 | 832.07 | 102.9 | 99.0 | 28,872 |
| Lepidium sativum | 24 | 0.75 | 739.09 | 299.6 | 91.2 | 14,372 |
| Brassicaceae lineage II | ||||||
| Cochlearia officinalis | 28 | 0.83 | 814.51 | 156.1 | 146.8 | 26,839 |
| Draba lactea | 32, 48 | 1.60 | 1,573.95 | 268.8 | 236.5 | 33,575 |
| Isatis tinctoria | 14, 28 | 0.84 | 830.57 | 129.9 | 123.4 | 30,803 |
| Pringlea antiscorbutica | 24 | 0.62 | 611.00 | 151.3 | 130.5 | 27,788 |
| Sisymbrium officinale | 14 | 0.83 | 815.81 | 198.7 | 183.6 | 38,819 |
| Stanleya pinnata | 14 | 0.87 | 853.09 | 264.6 | 205.6 | 32,009 |
| Brassicaceae lineage III | ||||||
| Hesperis matronalis | 24 | 1.87 | 1,844.03 | 307.9 | 114.2 | 16,251 |
Complete statistics on transcriptome sequencing and de novo assembly are provided in Supplemental Table 3.
Additional information on each species, including origin, life cycle, and their edible and medicinal uses is provided in Supplemental Table 1.
A list of completely sequenced Brassicaceae species included in this study is provided in Supplemental Table 2.
Based on Warwick and Al-Shehbaz (2006). For each species, most frequently observed chromosome number(s) is presented.
In this study, 1C content was estimated using 4′,6-diamidino-2-phenylindole nuclei staining, with B. napus as the reference sample. The genome sizes were interpolated from a standard curve created using DAPI staining data from seven additional Brassicaceae species with well-established genome sizes (see Methods).