Figure 3.
The Expression of HEN1 Is Upregulated by Light and Requires Photoreceptors and HY5.
Real-time qRT-PCR analysis of the mRNA level of HEN1 in etiolated wild type (Ler, Wassilewskija, and Col-0), hy5, and the light signaling mutants phyA (A), phyB (B), cry1 (C), phyA cry1 cry2 (D), cop1-4, cop1-6 (E), and hy5 hyh (F) on exposure to FR (1.13 Wm−2), R (18 μmol m−2 s−1), B (15 μmol m−2 s−1), and W (100 μmol m−2 s−1), respectively, at the indicated times. The expression of UBQ10 was an internal control. Except for the relative HEN1 level in (E) being 1000-multiplied mean values of 2−ΔCT (ΔCT = CT, HEN1 – CT, UBQ10) for clarity, the level of HEN1 at each time is normalized to that of etiolated seedlings (0 h). Data are mean ± sd from three biological replicates (each with three technical replicates).