Figure 6.

Impaired Root Growth of the Arabidopsis RidA Knockout, Its Exacerbation by Ser, and Reversal by Ile.

(A) Root growth of Col-0 (WT) and the RidA knockout (KO) after 7 d on half-strength MS medium containing 1% sucrose and various concentrations of Ser. Data are means and se for 10 to 18 replicate plants. For each strain, significant differences (Student’s t test) from growth without Ser are indicated by asterisks: *P < 0.05; **P < 0.01.

(B) Time course showing root growth of wild-type and knockout plants grown with 0.5 mM Ser. Data are means and se for 11 to 12 replicate plants.

(C) Images of wild-type and knockout plants grown for 7 d with or without 0.5 mM Ser.

(D) Reversal of growth phenotypes by Ile. Plants were cultured for 7 d in the presence of the indicated concentrations of Ser, with or without 0.05 mM Ile. Data are means and se for 10 to 18 replicate plants. Significant differences between plants grown with or without Ile are indicated as above. The growth defect in the absence of Ser was not overcome by 0.05 mM Val or Leu.

(E) Images of wild-type and knockout plants grown for 7 d with or without 0.05 mM Ile.