Figure 1.

KO of GAPCs Decreases Arabidopsis Seed Oil Accumulation.

(A) NAD-dependent GAPDH activity in developing seeds. Total protein was extracted from seeds in two developing stages (8 to 10 DAF and 12 to 14 DAF). Values are means ± sd (n = 3).

(B) Seed weight of wild-type and GAPC double KOs. Seed weight was average weight based on calculation of the total weight (∼3 mg) and total number of seeds. Values are means ± sd (n = 3).

(C) Seed oil content of wild-type and GAPC mutants. Seed oil content was analyzed after seeds were harvested and dried under room temperature for at least 2 weeks. Seed oil content was calculated as the percentage of oil over the seed weight. Values are means ± sd (n = 3).

(D) Oil content per seed. Values are means ± sd (n = 3).

“L” in (A) to (D) indicates significantly lower than the wild type (Student’s t test, P < 0.05).