Figure 2.

Increased Seed Oil Content in GAPC OE Seeds.

(A) Immunoblotting of overexpressed GAPC1 and GAPC2 in Arabidopsis seeds (>12 DAF). GAPCs were either fused with a FLAG tag (GAPC-OE) or with both YFP and FLAG tags (YFP-GAPC-OE) under the control of the cauliflower mosaic virus 35S promoter. Total protein was extracted from the seeds of T3 homozygous lines and the wild type was used as negative control. Total protein (10 μg) was separated on a 10% SDS-PAGE gel, transferred to a membrane, and blotted with anti-FLAG antibody. Two independent lines were examined for each transformation.

(B) GAPDH activity assay using total proteins extracted from developing seeds (12 to 14 DAF) of T3 lines. Values are means ± sd (n = 3).

(C) Seed oil analyses of GAPC OE seeds (T3). Seed oil content was calculated as the percentage of oil over the seed weight. Values are means ± sd (n = 3).

(D) Fatty acid composition was altered in GAPC OE seeds. Fatty acid composition was calculated as mol %. Values are means ± sd (n = 3).

(E) Fatty acid double bond index was increased in OE seeds. Fatty acid double bond index was calculated as DBI = mol % of unsaturated fatty acids × number of double bonds of each unsaturated fatty acid. Values are means ± sd (n = 3).

“H” and “L” in (B) to (E) indicate significantly higher and lower than the wild type, respectively (Student’s t test, P < 0.05).

[See online article for color version of this figure.]