Manipulation of GAPCs Alters the Expression Level of Genes
Involved in Oil Biosynthesis in Developing Seeds.
KO of GAPCs decreases
while OE of GAPCs
increases the expression level of genes involved in oil biosynthesis in
developing seeds. RNA was extracted from T2 developing seeds (12 to 14 DAF), and the transcript level was
determined by real-time PCR normalized to UBQ10. Values are
means ± sd (n = 3). “H” and
“L” indicate significantly higher and lower than the wild type
(Student’s t test, P < 0.05).