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Engineered BFA-Sensitive GNL1 Translocates from the Golgi Apparatus to the TGN.
Triple labeling of BFA-sensitive GNL1-YFP (green) (A), BFA-resistant GNOM-MYC (red) (B), and anti-COPI antibody (blue) (C) in BFA-treated root epidermal cells expressing functional BFA-sensitive GNL1-YFP and BFA-resistant GNOM-MYC lines. A merged image is shown in (D). Note the endosomal localization of BFA-sensitive GNL1-YFP, but the Golgi localization of BFA-resistant GNOM-MYC. Bar = 4 μm.
