Table 1. Characterization of TGN/EE and GA Distribution Patterns for Various Mutants.
Number of Analyzed GAs | Number of Highly Vesiculated TGN/EE Profiles | Number of Clustered Golgi | |
---|---|---|---|
Wild type | 36 | 3 (8.3%) | 2 (5.6%) |
van7 | 33 | 21 (63.6%) | 13 (39.4%) |
gnomR5 | 18 | 13 (72.2%) | 10 (55.5%) |
TGN/EE located within 150 to 200 nm from GAs (Golgi apparatus) were analyzed. Highly vesiculated TGN/EE profiles corresponded to TGN/EE with pronounced budding/fusing vesicles in single TEM sections. Numbers between parentheses represents percentage of GAs associated with highly vesiculated TGN/EE. “Clustered GA” is defined as the Golgi stacks located within 450 to 600 nm from each other. Numbers between parentheses represent percentage of clustered GAs.