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. 2014 Jul 17;26(7):3077–3089. doi: 10.1105/tpc.114.126441

Figure 1.

Figure 1.

Direct Interaction and Phosphorylation between CPK18 and MPK5 in Vitro.

(A) Recombinant His-CPK18 and His-MPK5 phosphorylated kinase-defective His-MPK5KR (K65R; labeled as KR) and His-CPK18DA (D178A; labeled as DA), respectively. The size difference between CPK18 and CPK18DA was due to autophosphorylation. Recombinant His-MPK5 purified from E. coli was capable of autophosphorylation and possessed the catalytic activity. Asterisks indicate bacterial protein contaminants with His-MPK5KR. CBB, Coomassie Brilliant Blue staining.

(B) MPK5 interacted with CPK18 in the GST pull-down assay. WB, immunoblotting.