Table 2. Kinetic Analysis of PDT and ADT Activity of the C. tepidum ADT Homolog.

Substrate	kcat (s−1)	Km (μM)	kcat/Km (mM−1 s−1)
Arogenate	5.4 ± 0.2	1544 ± 122	3.5 ± 0.2
Prephenate	0.27 ± 0.04	827 ± 87	0.32 ± 0.02

Kinetic parameters were obtained for arogenate (ADT activity) and prephenate (PDT activity) in 5- and 30-min reactions using 2 and 37.2 μg/mL recombinant enzyme, respectively. Data are means ± se (n = 3 independent experiments).