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Role of IFN-γ in STAT1 phosphorylations. INS-1E cells were cultured for 30 min or 2 h in the absence or presence of IL-1β and TNF-α with IFN-γ (3mix) or without IFN-γ (2mix) and with DRB (50 μM) (DRB50) as indicated before determination of a, b STAT1 phosphotyrosine 701 (pSTATtyr701) and c, d STAT1 phosphoserine 727 (pSTAT1ser727). Results are mean ± SEM (n = 3) with a representative experiment shown below bars. d *P < 0.01 versus 3 mix
