Fig. 3.

The effect of an aspA null mutation on central carbon metabolism. Metabolites in black typeface show an increase in relative abundance in the aspA mutant; white typeface a decrease in relative abundance; grey typeface no change and italicized typeface, not detected in either strain. *p value <0.05 between the C. jejuni NCTC 11168 (black bars) and aspA strain metabolite levels (open bars), displayed as a % of the total ion count with bin values depicted above. Aspartate and glutamate are 10.5 and 12.6 fold increased respectively in the aspA strain compared to the wild-type. Most central metabolites are lower in relative abundance in the aspA strain, exceptions being 2-oxoglutarate, a glutamate precursor, malate and citrate