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MRM transitions of all 29 metabolites (200 μM). Liquid chromatography was performed on an Agilent 1290 Infinity UHPLC system equipped with a Zorbax SB C-18 column (3.0 × 100 mm, 1.8 μm) at 25 °C column temperature. The mobile phases consisted of water and acetonitrile each containing 0.3 % HFBA and 0.5 % formic acid
