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. 2014 Aug 20;7:116. doi: 10.1186/s13068-014-0116-8

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© Sun et al.; licensee Springer 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Material balances during the synergistic treatment and enzymatic hydrolysis for the four pretreatment conditions. (a) Hydrothermal pretreatment at 160°C for 60 min combined with the alkali treatment, (b) hydrothermal pretreatment at 180°C for 30 min combined with the alkali treatment, (c) hydrothermal pretreatment at 180°C for 60 min combined with the alkali treatment, and (d) hydrothermal pretreatment at 200°C for 30 min combined with the alkali treatment.