Table 1.
Mutant numbers selected by ultrasound-mediated neomycin treatment of ZBY-3 spores a.
| Ultrasound (W) | Neomycin (mg/mL) | Treatment Time at 4 °C | Total | |||
|---|---|---|---|---|---|---|
| 3 h | 12 h | 24 h | 48 h | |||
| 200 | 200 | 4 | 1 | 1 | 2 | 8 |
| 400 | 1 | 2 | 1 | 0 | 4 | |
| Sum | 5 | 3 | 2 | 2 | 12 | |
| 400 | 200 | 1 | 0 | 2 | 4 | 7 |
| 400 | 2 | 1 | 3 | 5 | 11 | |
| Sum | 3 | 1 | 5 | 9 | 18 | |
| Total | 8 | 4 | 7 | 11 | 30 | |
a After ultrasound irradiation of the fresh ZBY-3 spore suspensions at the given ultrasound power and neomycin concentration, the spore suspensions were kept at 4 °C to treat the spores with neomycin. In the treatment period, each 100 μL of the spore suspensions was spread on PDA plates at the given treatment time and incubated at 28 °C for 3–5 days. Mutant colonies developed on the PDA plates were selected by single colony isolation during the incubation period to obtain mutants showing the numbers in this table.