Figure 4.

The expression of apoptosis-associated proteins in HepG2 cell treated with galaxamide and A5 for 72 h. A western blot was used to analyze whole cell lysates for caspase-3, caspase-9 and cleaved PARP expression after treatment with galaxamide and A5, and equal amounts of the protein were loaded onto each lane. One representative immunoblot from three independent experiments is presented. The fold change was calculated as the ratio of cleaved caspase-3, cleaved caspasse-9 and cleaved PARP in the different concentration of Galaxamide or A5 to their expression in the control cells, after normalization to tubulin in each lane. All data shown are representative of at least three separate studies.