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1H NMR spectra overlay of the main compounds and final C-2 spacer quaternary derivatives of the representative chitosan material (CS-i, 7%_DA): (A) chitosan mesylate salts (Mes-CS), 2i; (B) diTBDMS-CS, 3i; (C) N-(bromoacetyl)-3,6-di-O-TBDMS-chitosan (BrA-diTBDMS-CS), 4i; (D) TMA-CS, 6i; (E) PyA-CS, 8i.
