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. 2014 Aug 21;12(8):4635–4658. doi: 10.3390/md12084635

Table 2.

Antibacterial activity, hemolytic activity and cytotoxicity of the quaternary chitosan derivatives.

Compounds Structure S. aureus (ATCC 29213) E. coli (ATCC 25922) HC50 (μg/mL) Selectivity (HC50/MIC) EC50 (μg/mL)
MIC (μg/mL) MLC (μg/mL) MIC (μg/mL) MLC (μg/mL) S. aureus E. coli
TMC (15i) graphic file with name marinedrugs-12-04635-i003.jpg 8 64 256 256 6114 764 47.7 40
TMC (15ii) 32 32 64 64 6114 191 95.5 -
TMC (15iii) 4 4 64 64 6114 1528 95.5 -
TMC (15iv) 8 8 256 256 3072 764 47.7 10
TMC (15v) 32 32 256 1024 640 191 - -
TMA-CS (6i) graphic file with name marinedrugs-12-04635-i004.jpg 8 8 16,384 ≥32,768 ≥32,768 ≥4096 ≥2 26
TMA-CS (6ii) 8 8 16,384 16,384 ≥32,768 ≥4096 ≥2 -
TMA-CS (6iii) 32 32 16,384 16,384 ≥32,768 ≥1024 ≥2 -
TMA-CS (6iv) 32 32 ≥32,768 ≥32,768 ≥32,768 ≥1024 - 66
TMA-CS (6v) 128 128 ≥32,768 ≥32,768 ≥32,768 ≥256 - -
PyA-CS (8i) graphic file with name marinedrugs-12-04635-i005.jpg 8 1024 16,384 16,384 ≥32,768 ≥8192 ≥2 38
PyA-CS (8ii) 8 512 8192 8192 ≥32,768 ≥8192 ≥4 -
PyA-CS (8iii) 1024 1024 16,384 16,384 ≥32,768 ≥8 ≥2 -
PyA-CS (8iv) 512 1024 16,384 16,384 ≥32,768 ≥16 ≥2 12
PyA-CS (8v) 512 512 128 8192 ≥32,768 ≥64 ≥256 -
TMHA-CS (11i) graphic file with name marinedrugs-12-04635-i006.jpg 1024 2048 256 ≥32,768 ≥32,768 ≥32 ≥128 644
TMHA-CS (11ii) 2048 2048 512 16,384 ≥32,768 ≥16 ≥64 -
TMHA-CS (11iii) 1024 2048 128 ≥32,768 ≥32,768 ≥4 ≥256 -
TMHA-CS (11iv) 2048 4096 512 ≥32,768 ≥32,768 ≥8 ≥64 108
TMHA-CS (11v) 1024 4096 1024 ≥32,768 ≥32,768 ≥32 ≥32 -
PyHA-CS (13i) graphic file with name marinedrugs-12-04635-i007.jpg 4096 4096 ≥32,768 ≥32,768 ≥32,768 ≥8 - 4
PyHA-CS (13ii) 2048 2048 ≥32,768 ≥32,768 ≥32,768 ≥32 - -
PyHA-CS (13iii) 8192 8192 ≥32,768 ≥32,768 ≥32,768 ≥4 - -
PyHA-CS (13iv) 2048 2048 16,384 16,384 ≥32,768 ≥16 ≥2 18
PyHA-CS (13v) 2048 2048 ≥32,768 ≥32,768 ≥32,768 ≥16 - -

The antibacterial tests was done according the Clinical and Laboratory Standards Institute (CLSI) protocol (see Section 3.4.1). According to this procedure, a single dilution series was done for each compound, and gentamycin was used as a positive control. A difference of 1–2 dilutions is therefore not considered significant. The hemolysis measurements were also done in singlets for each concentration. The cytotoxicity measurements were carried out in triplicate, and the standard deviation varied from 10% to 22%.