Table 3.
Primers used for amplification of FlAlyA gene.
| Primer Name | Nucleotide Sequence (Corresponding Amino-Acid Sequence) |
|---|---|
| FlAly-1F | 5′-WSNRACNGCNAARATHGAYTGG-3′ |
| (SKTAKIDW) | |
| FlAly-2F | 5′-TGGWSNCAYTGGACNGTNACNGTNCC-3′ |
| (WSHWTVTVP) | |
| PL7-Rv | 5′-TANARNCCNGCYTTRAARTA-3′ |
| (YFKAGNY) | |
| InvFw1 | 5′-AATGAGAGGTACGTATGCTATTGACGAC-3′ |
| InvFw2 | 5′-GCGTTATTATTGCGCAAATTCACGG-3′ |
| InvRv1 | 5′-CAACAGACTTGTCTTTTGGGTCATCGTA-3′ |
| InvRv2 | 5′-GGATGCGATTTTATCCTCAGCATAATTT-3′ |
| FlAlyA-Fw | 5′-ACCAAAGTGGTAGAATAATAAAA-3′ |
| FlAlyA-Rv | 5′-ACACTTTAAAACAGATTAGCTAAACCG-3′ |
Amino-acid sequences used for designing of primers are shown in the parentheses. N, adenine or guanine or cytosine or thymine; R, adenine or guanine; Y, cytosine or thymine; H, adenine or cytosine or thymine; S, cytosine or guanine; and W, adenine or thymine.