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. 2014 Aug 18;37(8):613–619. doi: 10.14348/molcells.2014.0155

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The Korean Society for Molecular and Cellular Biology. All rights reserved.

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Fig. 1. — Expression and characterization of OM/tp fusion protein. (A) Schematic structure of pET30a-OM/tp recombinant plasmid. (B) SDS-PAGE and Western blot analysis of OM/tp recombinant protein. M, protein markers; lane 1, total bacterial protein without induction; lane 2, total bacterial protein after induction by IPTG (0.1 mM); lane 3, the supernatant from IPTG-induced total bacterial protein; lane 4, lysates of inclusion bodies from IPTG-induced total bacterial protein; lane 5, purified OM/tp recombinant protein; lane 6, Western blot analysis of purified OM/tp recombinant protein.