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Effect of OM/tp-NgR siRNA on axon regeneration of RGC. RGC were pre-incubated with forskolin (100 μM) for 6 h. Then the cells were treated with PBS (A), OM/tp (B), NgR siRNA (C), OM/tp-NgR siRNA (D), or OM/tp-nonspecific siRNA (E) and incubated for 72 h. Cells were immunostained with GAP-43 antibody and observed by laser scanning confocal microscopy. (F) The average axon length of the cells was measured by Image-Pro Plus 6.0 software. *p < 0.05 and **p < 0.01.
